先導(dǎo)編輯——基因編輯再升級(jí)

Scientists in the US have developed a powerful new genome editing system that could offer significantly more precision and efficiency than the current CRISPR standard.

美國科學(xué)家已研發(fā)出強(qiáng)大的新的基因組編輯系統(tǒng)，顯著提高目前CRISPR標(biāo)準(zhǔn)的精度和效率。

While CRISPR-Cas9 is a revolutionary medical technology that lay the modern foundations for editing genetic code – including variants associated with disease – there have long been concerns over its potential for imprecision.

雖然CRISPR-Cas9是革命性的醫(yī)療技術(shù)，為編輯包括與疾病相關(guān)的變異在內(nèi)的遺傳密碼奠定了現(xiàn)代基礎(chǔ)，但一直存在對其潛在的不精確性的擔(dān)憂。

Specifically, many fear that CRISPR-Cas9 editing carries the potential to introduce errors in the form of uncontrolled insertions and deletions in genetic code, called indels.

具體來說就是很多人害怕CRISPR-Cas9編輯有可能在被稱為索引的遺傳密碼中不受控制的插入和刪除信息從而導(dǎo)致錯(cuò)誤。

Researchers say the new system, dubbed 'prime editing' by its inventors at the Broad Institute of MIT and Harvard University, could change the game thanks to a new protein that enables high-precision edits of genetic targets.

研究人員說被麻省理工學(xué)院和哈佛大學(xué)的布羅德研究所的發(fā)明者稱為“先導(dǎo)編輯”的這一新系統(tǒng)能改變基因，有一種新型蛋白質(zhì)使研究人員可以對目標(biāo)基因進(jìn)行高精度編輯。

"A major aspiration in the molecular life sciences is the ability to precisely make any change to the genome in any location," says genome biologist David Liu from the the Broad Institute.

布羅德研究所的基因組生物學(xué)家David Liu說：“分子生命科學(xué)的一個(gè)主要目標(biāo)是能精確改變?nèi)魏挝恢玫幕蚪M。”

"We're not aware of another editing technology in mammalian cells that offers this level of versatility and precision with so few byproducts."

“我們不知道有其他對哺乳動(dòng)物細(xì)胞進(jìn)行編輯的技術(shù)能像這一技術(shù)一樣用途廣泛、精度高，而且副作用這么少。”

The basis of the new prime editing method is an enzyme called reverse transcriptase. The CRISPR system also uses an enzyme – Cas9 – to cut DNA strands, so that alternative genetic code can be inserted.

這一新的先導(dǎo)編輯方法的基礎(chǔ)是被稱為逆轉(zhuǎn)錄酶的酶，CRISPR系統(tǒng)也利用這種Cas9酶切割DNA鏈，插入替代遺傳密碼。

Now, thanks to reverse transcriptase, which is used in conjunction with Cas9 in prime editing, genomic editing has been upgraded again.

現(xiàn)在多虧有了逆轉(zhuǎn)錄酶，基因組編輯再次升級(jí)了。逆轉(zhuǎn)錄酶與Cas9一起被用于先導(dǎo)編輯。

In prime editing, a guide RNA called pegRNA guides a modified form of the Cas9 enzyme to snip only a single strand of DNA (preventing the double-strand breaks that can induce unintended disruptions).

先導(dǎo)編輯中名為pegRNA的引導(dǎo)RNA指引修改過的Cas9酶只剪斷一條DNA鏈(避免雙鏈斷裂導(dǎo)致意外中斷)。

After this, the reverse transcriptase enzyme directly copies edited genetic information contained in the pegRNA to the targeted genomic site.

然后逆轉(zhuǎn)錄酶直接將pegRNA中編輯過的基因信息復(fù)制到基因組的目標(biāo)位置。